Flight Mechanics Modeling and Post-Flight Analysis of ADEPT SR-1

Sounding Rocket One (SR-1), the first flight test of the Adaptable Deployable Entry and Placement Technology (ADEPT), was performed on Sept. 12, 2018. ADEPT is a deployable aeroshell that can be stowed during launch and then opened after launch to increase the drag area of the spacecraft when entering into a planetary atmosphere. The main objectives of the SR-1 flight test were to demonstrate that the ADEPT vehicle can be opened exo-atmospherically and to characterize the stability of the vehicle during atmospheric flight. The SR-1 test vehicle was a 0.7 m diameter 70 degree half-angle, faceted, sphere-cone, which was the primary payload on an UP Aerospace Spaceloft (SL) launch vehicle from the White Sands Missile Range (WSMR). ADEPT successfully separated from the spent booster in its stowed configuration, opened above 100 km altitude, and then landed in the deployed configuration within WSMR. The flight mechanics of the vehicle was modeled pre-flight for performance and range safety predictions. This paper describes the pre-flight ADEPT trajectory simulation and how the flight data compared with the predictions from the simulations

Subsonic Dynamic Testing of a Subscale ADEPT Entry Vehicle

The Adaptive Deployable Entry and Placement Technology (ADEPT) is a mechanically-deployed entry system. A sounding rocket test flight of an ADEPT vehicle, known as ADEPT SR-1, was conducted in September 2018. Prior to this sounding rocket test, an investigation was performed using the NASA Langley Research Center 20-ft Vertical Spin Tunnel (VST) to assess the free-flight dynamic characteristics of ADEPT SR-1 at subsonic speeds. The model of ADEPT SR-1 for this VST test was fabricated at 50-percent geometric scale, with dynamically scaled mass properties (Froude scaled) to represent full-scale flight at an altitude of 1.2 km above sea level. The subsonic dynamic characteristics of ADEPT SR-1 were of interest prior to the sounding rocket test because of payload recovery considerations. At low roll rates the model was found to have acceptable dynamic characteristics. It was statically stable in pitch and yaw, exhibiting limit cycle pitch/yaw oscillations of no greater than 20 degrees (the angle between the models longitudinal axis and nadir). The model was able to recover from large upsets in pitch and yaw, although if sufficiently provoked it tumbled. Damping in roll was low. At high roll rates the pitch and yaw oscillations grew in magnitude and rate. This behavior was also observed during the sounding rocket flight test

Parallelization of a Six Degree of Freedom Entry Vehicle Trajectory Simulation Using OpenMP and OpenACC

The art and science of writing parallelized software, using methods such as Open Multi-Processing (OpenMP) and Open Accelerators (OpenACC), is dominated by computer scientists. Engineers and non-computer scientists looking to apply these techniques to their project applications face a steep learning curve, especially when looking to adapt their original single threaded software to run multi-threaded on graphics processing units (GPUs). There are significant changes in mindset that must occur; such as how to manage memory, the organization of instructions, and the use of if statements (also known as branching). The purpose of this work is twofold: 1) to demonstrate the applicability of parallelized coding methodologies, OpenMP and OpenACC, to tasks outside of the typical large scale matrix mathematics; and 2) to discuss, from an engineers perspective, the lessons learned from parallelizing software using these computer science techniques. This work applies OpenMP, on both multi-core central processing units (CPUs) and Intel Xeon Phi 7210, and OpenACC on GPUs. These parallelization techniques are used to tackle the simulation of thousands of entry vehicle trajectories through the integration of six degree of freedom (DoF) equations of motion (EoM). The forces and moments acting on the entry vehicle, and used by the EoM, are estimated using multiple models of varying levels of complexity. Several benchmark comparisons are made on the execution of six DoF trajectory simulation: single thread Intel Xeon E5-2670 CPU, multi-thread CPU using OpenMP, multi-thread Xeon Phi 7210 using OpenMP, and multi-thread NVIDIA Tesla K40 GPU using OpenACC. These benchmarks are run on the Pleiades Supercomputer Cluster at the National Aeronautics and Space Administration (NASA) Ames Research Center (ARC), and a Xeon Phi 7210 node at NASA Langley Research Center (LaRC)

Adaptive Control Allocation for Powered Descent Vehicles

The following work details a study into real-time failure adaptive control allocation method for powered descent vehicle systems. The motivation for this work is to enable future human and robotic missions utilizing a powered descent system to tolerate engine failures in flight without the loss of crew or assets. This study is conducted using a six degree-of-freedom trajectory simulation of a PDV (Powered Descent Vehicle) experiencing either a loss of thrust or an engine stuck full on failure scenario. Sequential least squares in the frequency domain is used on-board to process inertial measurement unit (IMU) data and generate an estimate of the PDV plant model, which is then fed to the guidance and control system. Data used by the sequential least squares method is generated from an in-flight maneuver. The work herein focuses on determining a maneuver that is least impactful to the PDV trajectory and enables a suitable plant model estimate. A 1.5-second-long maneuver with an amplitude of 5 percent throttle is determined to provide suitable data for the sequential least squares method to estimate a plant model. A PDV implementing this method can adapt to a single engine failure and continue to reach its touchdown conditions

In an in vitro model of human tuberculosis, monocyte-microglial networks regulate matrix metalloproteinase-1 and -3 gene expression and secretion via a p38 mitogen activated protein kinase-dependent pathway.

BACKGROUND: Tuberculosis (TB) of the central nervous system (CNS) is characterized by extensive tissue inflammation, driven by molecules that cleave extracellular matrix such as matrix metalloproteinase (MMP)-1 and MMP-3. However, relatively little is known about the regulation of these MMPs in the CNS. METHODS: Using a cellular model of CNS TB, we stimulated a human microglial cell line (CHME3) with conditioned medium from Mycobacterium tuberculosis-infected primary human monocytes (CoMTb). MMP-1 and MMP-3 secretion was detected using ELISAs confirmed with casein zymography or western blotting. Key results of a phospho-array profile that detects a wide range of kinase activity were confirmed with phospho-Western blotting. Chemical inhibition (SB203580) of microglial cells allowed investigation of expression and secretion of MMP-1 and MMP-3. Finally we used promoter reporter assays employing full length and MMP-3 promoter deletion constructs. Student's t-test was used for comparison of continuous variables and multiple intervention experiments were compared by one-way ANOVA with Tukey's correction for multiple pairwise comparisons. RESULTS: CoMTb up-regulated microglial MMP-1 and MMP-3 secretion in a dose- and time-dependent manner. The phospho-array profiling showed that the major increase in kinase activity due to CoMTb stimulation was in p38 mitogen activated protein kinase (MAPK), principally the α and γ subunits. p38 phosphorylation was detected at 15 minutes, with a second peak of activity at 120 minutes. High basal extracellular signal-regulated kinase activity was further increased by CoMTb. Secretion and expression of MMP-1 and MMP-3 were both p38 dependent. CoMTb stimulation of full length and MMP-3 promoter deletion constructs demonstrated up-regulation of activity in the wild type but a suppression site between -2183 and -1612 bp. CONCLUSIONS: Monocyte-microglial network-dependent MMP-1 and MMP-3 gene expression and secretion are dependent upon p38 MAPK in tuberculosis. p38 is therefore a potential target for adjuvant therapy in CNS TB

Examining the Role of Chloride Ligands on Defect Removal in Imperfectly Attached Semiconductor Nanocrystals for 1D and 2D Attachment Cases

Semiconducting, core-shell nanocrystals (NCs) are promising building blocks for the construction of higher dimensional artificial nanostructures using oriented attachment. However, the assembly and epitaxial attachment steps critical to this construction introduce disorder and defects which inhibit the observation of desirable emergent electronic phenomena. Consequently, understanding defect formation and remediation in these systems as a function of dimensionality is a crucial step to perfecting their synthesis. In this work, we use in situ high resolution transmission electron microscopy to examine the role of chloride ligands as remediator agents for imperfect attachment interfaces between CdSe/CdS core-shell NCs for both 1D and 2D attachment cases. In the 1D case, we find that the presence of chloride additives in imperfectly attached NC dimers can result in defect removal speeds nearly twice as large as those found in their plain, non-chloride treated counterparts. However, when we increased the dimensionality of the system and examined 2D NC arrays, we found no statistically significant difference in attachment interface quality between the chloride and non-chloride treated samples. We propose that this discongruity arises from fundamental differences between 1D and 2D NC attachment and discuss synthetic guidelines to inform future nanomaterial superlattice design.Comment: 35 pages, 6 figures, work conducted at the University of California, Berkele

Mycobacterium tuberculosis-infected human monocytes down-regulate microglial MMP-2 secretion in CNS tuberculosis via TNFα, NFκB, p38 and caspase 8 dependent pathways

Tuberculosis (TB) of the central nervous system (CNS) is a deadly disease characterized by extensive tissue destruction, driven by molecules such as Matrix Metalloproteinase-2 (MMP-2) which targets CNS-specific substrates. In a simplified cellular model of CNS TB, we demonstrated that conditioned medium from Mycobacterium tuberculosis-infected primary human monocytes (CoMTb), but not direct infection, unexpectedly down-regulates constitutive microglial MMP-2 gene expression and secretion by 72.8% at 24 hours, sustained up to 96 hours (P < 0.01), dependent upon TNF-α. In human CNS TB brain biopsies but not controls the p38 pathway was activated in microglia/macrophages. Inhibition of the p38 MAP kinase pathway resulted in a 228% increase in MMP-2 secretion (P < 0.01). In contrast ERK MAP kinase inhibition further decreased MMP-2 secretion by 76.6% (P < 0.05). Inhibition of the NFκB pathway resulted in 301% higher MMP-2 secretion than CoMTb alone (P < 0.01). Caspase 8 restored MMP-2 secretion to basal levels. However, this caspase-dependent regulation of MMP-2 was independent of p38 and NFκB pathways; p38 phosphorylation was increased and p50/p65 NFκB nuclear trafficking unaffected by caspase 8 inhibition. In summary, suppression of microglial MMP-2 secretion by M.tb-infected monocyte-dependent networks paradoxically involves the pro-inflammatory mediators TNF-α, p38 MAP kinase and NFκB in addition to a novel caspase 8-dependent pathway

Assembly of 809 whole mitochondrial genomes with clinical, imaging, and fluid biomarker phenotyping

INTRODUCTION: Mitochondrial genetics are an important but largely neglected area of research in Alzheimer's disease. A major impediment is the lack of data sets. METHODS: We used an innovative, rigorous approach, combining several existing tools with our own, to accurately assemble and call variants in 809 whole mitochondrial genomes. RESULTS: To help address this impediment, we prepared a data set that consists of 809 complete and annotated mitochondrial genomes with samples from the Alzheimer's Disease Neuroimaging Initiative. These whole mitochondrial genomes include rich phenotyping, such as clinical, fluid biomarker, and imaging data, all of which is available through the Alzheimer's Disease Neuroimaging Initiative website. Genomes are cleaned, annotated, and prepared for analysis. DISCUSSION: These data provide an important resource for investigating the impact of mitochondrial genetic variation on risk for Alzheimer's disease and other phenotypes that have been measured in the Alzheimer's Disease Neuroimaging Initiative samples

Holographic Normal Ordering and Multi-particle States in the AdS/CFT Correspondence

The general correlator of composite operators of N=4 supersymmetric gauge field theory is divergent. We introduce a means for renormalizing these correlators by adding a boundary theory on the AdS space correcting for the divergences. Such renormalizations are not equivalent to the standard normal ordering of current algebras in two dimensions. The correlators contain contact terms that contribute to the OPE; we relate them diagrammatically to correlation functions of compound composite operators dual to multi-particle states.Comment: 18 pages, one equation corr., further comments and refs. adde

Bis(\u3cem\u3eN\u3c/em\u3e-amidinohydrazones) and \u3cem\u3eN\u3c/em\u3e-(amidino)-\u3cem\u3eN\u3c/em\u3e\u27-aryl-bishydrazones: New Classes of Antibacterial/Antifungal Agents

The emergence of multidrug-resistant bacterial and fungal strains poses a threat to human health that requires the design and synthesis of new classes of antimicr obial agents. We evaluated bis(N-amidinohydrazones) and N-(amidino)-N\u27-aryl-bishydrazones for their antibacterial and antifungal activities against panels of Gram-positive/Gram-negative bacteria as well as fungi. We investigated their potential to develop resistance against both bacteria and fungi by a multi-step, resistance-selection method, explored their potential to induce the production of reactive oxygen species, and assessed their toxicity. In summary, we found that these compounds exhibited broad-spectrum antibacterial and antifungal activities against most of the tested strains with minimum inhibitory concentration (MIC) values ranging from \u3c 0.5- \u3e 500 μM against bacteria and 1.0- \u3e 31.3 μg/mL against fungi; and in most cases, they exhibited either superior or similar antimicrobial activity compared to those of the standard drugs used in the clinic. We also observed minimal emergence of drug resistance to these newly synthesized compounds by bacteria and fungi even after 15 passages, and we found weak to moderate inhibition of the human Ether-à-go-go-related gene (hERG) channel with acceptable IC50 values ranging from 1.12-3.29 μM. Overall, these studies sh ow that bis(N-amidinohydrazones) and N-(amidino)-N\u27-aryl-bishydrazones are potentially promising scaffolds for the discovery of novel antibacterial and antifungal agents